PTC Extraction and Amplification Kit with 0.2-mL Tubes (with prepaid coupon)

PTC Extraction and Amplification Kit with 0.2-mL Tubes (with prepaid coupon)

PTC Extraction and Amplification Kit with 0.2-mL Tubes (with prepaid coupon)

211377
Regular price $281.00
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Advanced - For experienced high school and college classes; requires some basic technical skill.

This kit explores the molecular basis of the inherited ability to taste the bitter chemical phenylthiocarbamide (PTC), which was first described in the 1930s by Cold Spring Harbor Laboratory scientist Albert Blakeslee. Students determine their ability to taste PTC using taste paper. They then use safe saline mouthwash and Chelex® extraction to obtain a sample of their own DNA and amplify a 221-nucleotide region of the PTC taste receptor gene. The 2 alleles differ by a single nucleotide, so restriction digestion of the amplified product followed by gel electrophoresis effectively differentiates the 2 alleles. One allele strongly correlates to the ability to taste PTC. After scoring their SNP genotypes, students determine how well the SNP genotypes actually correlate to tasting.

Kit provides materials for 25 PCR reactions and restriction digests. Kit also includes a prepaid coupon to request perishable materials later at your convenience. Contact us or return the coupon to request delivery of perishables.

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Merlan Scientific warrants that each product will be free of defects in materials and workmanship for a period set forth in its published user manuals for its system products and for a period of one (1) year for its component products. The warranty commences on the date the product is shipped by Merlan Scientific. Merlan Scientifics’ sole liability and responsibility under this warranty is to repair or replace any defective product, which is returned to it by the buyer, and which Merlan Scientific determines qualifies for repair or replacement under the terms of the applicable warranty. The product returned to Merlan Scientific for warranty service will be shipped to Merlan Scientific at the buyers expense and will be returned to buyer at Merlan Scientifics’ expense. In no event shall Merlan Scientific be responsible under its warranty for any defect, which is caused by negligence, misuse or mistreatment of a product, or for any unit, which has been altered or modified in any way. The warranty of repaired and replacement products shall terminate with the original warranty of the product.

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