Carolina Investigations® for Use with AP® Chemistry: Spectrophotometric Analysis of Food Dyes Kit

Carolina Investigations® for Use with AP® Chemistry: Spectrophotometric Analysis of Food Dyes Kit

Carolina Investigations® for Use with AP® Chemistry: Spectrophotometric Analysis of Food Dyes Kit

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Determine the concentration of food dyes in powdered drink mixes using Beer-Lambert's law. Teach this laboratory experience using either the guided activity or inquiry activity. In the guided activity, students prepare solutions of known concentration for each of 2 food dyes, measure each solution's absorbance, and graph a calibration curve for each food dye. Students then use their data and curve to determine the concentration of food dye in unknown solutions containing a single food dye.

The inquiry activity allows students to design a procedure to determine the concentration of each food dye in its unknown solution. Students must choose the concentrations of the standard solutions that will allow them to interpolate the concentration of food dye in the drink mix and prepare the solutions. It also provides opportunity for students to present their experiment's design and results, reinforcing the practice of communicating findings. Detailed preparation and procedure notes guide you through leading a successful inquiry exercise, including a suggested rubric for assessing student performance.

Both activities include assessment questions that follow the AP® Chemistry Exam free-response question format. In the assessment, students are given absorbance data for a complex ion, asked to identify the wavelength of maximum absorbance, plot provided data to generate a calibration curve, and determine the concentration of the complex ion.

This kit provides the following AP® Chemistry experiences: design a procedure to collect data; analyze data; perform error analysis; plot calibration curves, and interpolate data from the curves. Because the experiment uses food dyes rather than thiocyanate salts, it is safer and generates no hazardous waste. Materials are sufficient for 30 students working in teams of 3. Digital teacher's manual, included FREE with kit purchase, is a 12-month eBook license to the Spectrophotometric Analysis of Food Dyes teacher's manual. You can also access the digital student guide for this kit for free at Carolina Science Online®.

AP® is a trademark registered and/or owned by the College Board®, which was not involved in the production of, and does not endorse, these products.

Name Include Note
Wash Bottles of Distilled Water Needed, Not included
Graduated Cylinders, 100 mL Needed, Not included
Analytical Balance (accurate to 0.001 g) Needed, Not included
Spectrophotometer or Colorimeter Needed, Not included
Volumetric Flasks, 100 mL Needed, Not included
Volumetric Flasks, 500 mL Needed, Not included
Red Dye No. 40 Powder, 2 g Included
Crystal Light® Lemonade Packest, Single Included
Yellow Dye No. 5 Powder, 2 g Included
Beakers, 100 mL Needed, Not included
Pipet Bulbs or Pipet Pumps Needed, Not included
Strawberry Kool-Aid® Packets, Single Included
Pink Lemonade Kool-Aid® Packets, Single Included
Measuring Pipets, 10 mL Needed, Not included
Teacher's Manual and Reproducible Student Guide Included
Cuvettes Needed, Not included
Kimwipes® or Paper Towels Needed, Not included
Marking Pencils or Pens Needed, Not included
Access to Distilled or Deionized Water Needed, Not included
Bottles with Cap, 2 L (may use clean soda bottles) Needed, Not included
Drinking Water, 500 mL Needed, Not included
Test Tubes Needed, Not included
Test Tube Racks Needed, Not included

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